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Anatrace
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Thermo Fisher
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Gold Biotechnology Inc
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Enzo Biochem
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Anatrace
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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: The cyanobacterial protoporphyrinogen oxidase HemJ is a new b -type heme protein functionally coupled with coproporphyrinogen III oxidase
doi: 10.1074/jbc.RA118.003441
Figure Lengend Snippet: Separation of the purified HemJ.f protein by size-exclusion chromatography. A, the HemJ.f pulldown obtained from the ΔPSI genetic background was loaded on a size-exclusion chromatography column, and eluted proteins/complexes were detected by absorbance 280 and 415 nm. Positions of standards are shown at the top of the graph: PSII[2], PSII dimer (600 kDa); PSII[1], PSII monomer (300 kDa); β-AM, β-amylase (200 kDa); ADH, alcohol dehydrogenase (150 kDa); BSA, BSA (66 kDa); DDM, micelle of dodecyl-β-maltoside; 3xFLAG, 3× FLAG peptide used to elute HemJ.f from the anti-FLAG-M2 agarose resin. B, absorption spectrum of 9.4 ml size-exclusion chromatography fraction was recorded by a HPLC diode-array detector.
Article Snippet: For the isolation of HemJ.f protein, the membrane fraction was resuspended in the thylakoid buffer and solubilized for 1 h at 10 °C with 1.5%
Techniques: Purification, Size-exclusion Chromatography